胶原酶II Collagenase from Clostridium histolyticum 
release of physiologically active rat epididymal adipocytes tested, Type II, ≥125 CDU/mg solid (CDU = collagendigestion units), 0.5-5.0 FALGPA units/mg solid  

胶原酶II Collagenase from Clostridium histolyticum suitability

          release of physiologically active rat epididymal adipocytes tested

 

胶原酶II Collagenase from Clostridium histolyticum   storage temp. −20°C

胶原酶II  Analysis Note  

Also contains clostripain, nonspecific neutral protease, and tryptic activities.

 

胶原酶II  Other Notes  

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胶原酶IIUnit Definition  

胶原酶IIOne collagen digestion unit liberates peptides from collagen equivalent in ninhydrin color to 1.0?μmole of leucine in 5?hr at pH 7.4 at 37 °C in the presence of calcium ions.
One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions.
One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0?μmole tyrosine per 5?hr at pH?7.5 at 37?°C.
胶原酶IIOne clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH?7.6 at 25?°C in the presence of DTT.