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产品名称: sc-25224原装现货
产品型号: sc-25224
产品展商: 上海博蕴生物科技有限公司
简单介绍
SANTA CRUZ BIOTECHNOLOGY, INC.The Power to QuestionCD30 (S-20): sc-25224原装现货CD30 (S-20): sc-25224原装现货goat polyclonal IgG, 200 µg/ml epitope mapping within an extracellular domain of CD30 of mouse origin recommended for detection of CD30 of mouse origin by WB, IF and ELISA,cross-reactive with CD30 short form blocking peptide, sc-25224 P


sc-25224原装现货的详细介绍

BACKGROUND  CD30 (S-20): sc-25224原装现货
The tumor necrosis factor (TNF) receptor family is composed of several type I
integral membrane glycoproteins that exhibit homology in their cystine-rich
extracellular domains. Members of this family include FAS, OX40, CD27 and
CD30. Ligands for these receptors are often type II transmembrane glycoproteins,
as is the case for CD27 and CD30. CD27 homodimeric lymphocytespecific
surface antigen present on T and B lymphocytes. Activation of the
CD3 complex via the T cell receptor for antigen leads to an increase in CD27
expression. Together, CD27 and its ligand, CD27L, generate co-stimulatory
signals required for complete T cell activation. CD30 is a surface marker for
neoplastic cells of the Hodgkin’s lymphoma and related hematologic malignancies.
CD30L has been shown to enhance the proliferation of the Hodgkin’s
cell line HDLM-2, but exerts antiproliferative effects on large cell anaplastic
lymphoma cell lines.  CD30 (S-20): sc-25224原装现货
REFERENCES  CD30 (S-20): sc-25224原装现货
1. Smith, C.A., et al. 1993. CD30 antigen, a marker for Hodgkin’s lymphoma,
is a receptor whose ligand defines an emerging family of cytokines with   CD30 (S-20): sc-25224原装现货
homology to TNF. Cell 73: 1349-1360.  CD30 (S-20): sc-25224原装现货
2. Armitage, R.J. 1994. Tumor necrosis factor receptor superfamily members
and their ligands. Curr. Opin. Immunol. 6: 407-413.    CD30 (S-20): sc-25224原装现货
3. Hintzen, R.Q., et al. 1994. CD27: marker and mediator of T-cell activation.   CD30 (S-20): sc-25224原装现货
Immunol. Today 15: 307-311.    CD30 (S-20): sc-25224原装现货
4. Gruss, H.J., et al. 1995. Tumor necrosis factor ligand superfamily: involvement
in the pathology of malignant lymphomas. Blood 85: 3378-3404.    CD30 (S-20): sc-25224原装现货
5. Lens, S.M., et al. 1995. CD27-CD70 interaction: unravelling its implication
in normal and neoplastic B-cell growth. Leuk. Lymphoma 18: 51-59.    CD30 (S-20): sc-25224原装现货
6. Wendtner, C.M., et al. 1995. CD30 ligand signal transduction involves
activation of a tyrosine kinase and of mitogen-activated protein kinase in
a Hodgkin’s lymphoma cell line. Cancer Res. 55: 4157-4161.    CD30 (S-20): sc-25224原装现货
7. Bowen, M.A., et al. 1996. Structure and expression of murine CD30 and
its role in cytokine production. J. Immunol. 156: 442-449.    CD30 (S-20): sc-25224原装现货
CHROMOSOMAL LOCATION  CD30 (S-20): sc-25224原装现货
Genetic locus: TNFRSF8 (human) mapping to 1p36; Tnfrsf8 (mouse) mapping
to 4 E1.    CD30 (S-20): sc-25224原装现货
SOURCE  CD30 (S-20): sc-25224原装现货
CD30 (S-20) is an affinity purified goat polyclonal antibody raised against a
peptide mapping within an extracellular domain of CD30 of mouse origin.
PRODUCT    CD30 (S-20): sc-25224原装现货
Each vial contains 200 μg IgG in 1.0 ml of PBS with < 0.1% sodium azide and
0.1% gelatin.    CD30 (S-20): sc-25224原装现货
Blocking peptide available for competition studies, sc-25224 P, (100 μg peptide
in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA).
RESEARCH USE    CD30 (S-20): sc-25224原装现货
For research use only, not for use in diagnostic procedures.
APPLICATIONS    CD30 (S-20): sc-25224原装现货
CD30 (S-20) is recommended for detection of CD30 of mouse origin by
Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000),
immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and
solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000); non
cross-reactive with CD30 short form.    CD30 (S-20): sc-25224原装现货
Suitable for use as control antibody for CD30 siRNA (m): sc-37244.
Molecular Weight of CD30: 120 kDa.    CD30 (S-20): sc-25224原装现货
RECOMMENDED SECONDARY REAGENTS  CD30 (S-20): sc-25224原装现货
To ensure optimal results, the following support (secondary) reagents are
recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020
(dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey
anti-goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™
Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:
sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluorescence:
use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100-
1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400)
with UltraCruz™ Mounting Medium: sc-24941.    CD30 (S-20): sc-25224原装现货
STORAGE  CD30 (S-20): sc-25224原装现货
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
PROTOCOLS    CD30 (S-20): sc-25224原装现货
See our web site at www.scbt.com or our catalog for detailed protocols
and support products.    CD30 (S-20): sc-25224原装现货

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